Mapping of posttranslational modifications

Identification (and quantification) of protein PTMS by LC-MS/MS from tissues, cells, isolated organelles, immunoprecipitates or purified proteins after protease digestion.

Ubiquitinylation

MSL2 Combines Sensor and Effector Functions in Homeostatic Control of the Drosophila Dosage Compensation Machinery. Villa R, Forné I, Müller M, Imhof A, Straub T, Becker PB. Mol Cell. 2012 Oct 16. doi:pii: S1097-2765(12)00788-5. 10.1016/j.molcel.2012.09.012.

Acetylation

Circadian acetylome reveals regulation of mitochondrial metabolic pathways. Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P. Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. doi: 10.1073/pnas.1217632110

Histone Modifications

DOT1A-dependent H3K76 methylation is required for replication regulation in Trypanosoma brucei. Gassen A, Brechtefeld D, Schandry N, Arteaga-Salas JM, Israel L, Imhof A and Janzen CJ.  Nucleic Acids Research, 2012, 1–10 doi:10.1093/nar/gks801

Developmental Regulation of N-terminal H2B Methylation in Drosophila Melanogaster. Villar-Garea A, Forné I, Vetter I, Kremmer E, Thomae A, Imhof A. Nucleic Acids Res. 2012 Feb;40(4):1536-49

Sequential establishment of marks on soluble histones H3 and H4. Alvarez F, Munoz F, Schilcher P, Imhof A, Almozuni G, and Loyola A. JBC 2011 286 (20) 17714–17721

Establishment of Histone Modifications after Chromatin Assembly. Scharf AN, Barth TK, Imhof A. Nucleic Acids Res. 2009 Jun 18.