Zentrallabor für Proteinanalytik (Protein Analysis Unit)
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Quantification of Proteins

 

Quantification (and identification) by LC-MS/MS after protease digestion of labelled/unlabelled proteins

Label Free

Stepwise evolution of essential centromere function in a Drosophila neogene. Ross BD, Rosin L, Thomae AW, Hiatt MA, Vermaak D, de la Cruz AF, Imhof A, Mellone BG, Malik HS. Science. 2013 Jun 7;340(6137):1211-4.

A pair of centromeric proteins mediates reproductive isolation in Drosophila species. Thomae AW, Schade GOM, Padeken J, Borath M, Vetter I, Kremmer E, Heun P, Imhof A. Dev Cell 2013 Nov 13. doi:pii: S1534-5807(13)00578-9. 10.1016/j.devcel.2013.10.001.

Absolute Quantitation

Characterization of the insertase for β-barrel proteins of the outer mitochondrial membrane. Klein A, Israel L, Lackey SW, Nargang FE, Imhof A, Baumeister W, Neupert W, Thomas DR. J Cell Biol. 2012 Nov 12;199(4):599-611

SILAC

Role of the AAA protease Yme1 in folding of proteins in the intermembrane space of mitochondria. Schreiner B, Westerburg H, Forné I, Imhof A, Neupert W, Mokranjac D. Mol Biol Cell. 2012 Nov;23(22):4335-46.